Molecular Formula: C19H42BrN
Molecular Weight: 364.5
Melting Point: 237-243 C1
Critical Micelle Concentration (CMC): 0.92 to 1.0 mM
(water)2,3
Aggregation number: 61 (water, 25 C),
169 (13 mM KBr)2,4
pH: 6.0-7.5 (0.1 M H2O, 0 °C).5
Hexadecyltrimethylammonium bromide is a bactericidal,
cationic detergent. Its activity is neutralized by soaps
and anionic detergents (i.e., SDS). Trimethylammonium
bromide compounds form insoluble complexes with
SDS.3 It is active at alkaline pH against both Gram-
positive and Gram-negative organisms.6
CTAB has been used for the isolation of plant high
molecular weight DNA (by a rapid method)7 as well as
plant DNA for use in PCR analysis.8,9 It has been used
to precipitate nucleic acids.7,8,10,11
CTAB provides enhancement of Concanavalin A
mediated agglutination.12 It has been used for the
determination of protein molecular weights in
electrophoretic systems13,14 and for determination of
critical micelle concentration of detergents.15 CTAB has
also been used as a titrant for potentiometric titration of
perchlorate16 and as a phase-transfer catalyst in
reduction of arenes and heterocyclic compounds.
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