Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Fast Green FCF has been used for in staining of menisci from rabbit and of osteochondral tissues.
Biochem/physiol Actions
Fast Green FCF can be considered as a safe stain to detect tumor. In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.
General description
Fast Green FCF is one of the triarylmethane dyes, that is biocompatible. This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics.
Packaging
5, 25, 100 g in glass bottle
