HBsAg ELISA (Blood Banking)

HBsAg Microwell ELISA is an in vitro enzyme immunoassay for the qualitative detection of Hepatitis B surface antigen (HBsAg) in human serum or plasma. It is based on a direct, non-competitive, solid-phase enzyme immunoassay principle using horseradish peroxidase (HRP) as the marker enzyme. After incubation and wash steps, colour develops using TMB substrate, the reaction is stopped, and absorbance is read at 450 nm (reference 630 nm) within 15 minutes of adding stop solution (as per IFU).

For general background on hepatitis B and HBsAg testing, see:

• Pack size: 96 tests
• Storage (kit): 2°C to 8°C

Key features

• Qualitative detection of HBsAg in human serum or plasma
• Direct, non-competitive, solid-phase microwell ELISA principle
• Read at 450 nm with 630 nm reference wavelength
• Includes positive and negative controls
• Analytical sensitivity 0.4 PEIU/ml, Diagnostic sensitivity 100%, Diagnostic specificity 100%